Review



2x blue native page sample buffer  (Sangon Biotech)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Sangon Biotech 2x blue native page sample buffer
    Diminished AQP4 expression in NOTCH3‐R170C astrocyte. (A–C) Brain cells of NOTCH3‐R170C and ‐WT mice (16 weeks of age) were subjected to single cell RNA sequencing (scRNAseq). (A) Left: Merged Uniform manifold approximation and projection (UMAP) plot representing 15 color‐coded cell clusters identified in the combined single‐cell transcriptomes. Cluster names were manually assigned. Right: Feature plots of Notch3 . (B) Expression of Notch3 among brain cells. (C) Violin plots of Notch3 and Aqp4 expression in astrocytes. (D) Brain protein of NOTCH3‐R170C or ‐WT mice (24 weeks of age) was subjected to western blot to evaluate AQP4 expression. N = 3 in each group. ** p < 0.01; by Student's t test (mean ± SEM). (E, F) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were prepared. (E) Aqp4 mRNA level was evaluated with RT‐PCR. (F) AQP4 protein expression in whole cell and plasma membrane of astrocytes was assessed with western blot. Experiments were repeated for three times. * p < 0.05; by Student's t test. Difference of means (± SEM) is displayed. (G) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were subjected to <t>BN</t> <t>page</t> and then 2D SDS page to evaluate the orthogonal arrays of particles (OAPs) of AQP4. (H) Coronal brain sections of NOTCH3‐R170C or ‐WT mice (24 weeks of age) were subjected to immunostaining of GFAP (green) and AQP4 (red). The AQP4 polarization was calculated by AQP4 perivascular endfeet area. White arrowheads indicated the depolarized AQP4 distribution. N = 6 in NOTCH3‐WT group and N = 5 in NOTCH3‐R170C group. * p < 0.05; by Student's t test (mean ± SEM).
    2x Blue Native Page Sample Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2x+blue+native+page+sample+buffer/2x+blue+native+page+sample+buffer/pmc11760990-160-9-15
    Average 90 stars, based on 1 article reviews
    2x blue native page sample buffer - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "NOTCH3 Mutation Causes Glymphatic Impairment and Promotes Brain Senescence in CADASIL"

    Article Title: NOTCH3 Mutation Causes Glymphatic Impairment and Promotes Brain Senescence in CADASIL

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.70140

    Diminished AQP4 expression in NOTCH3‐R170C astrocyte. (A–C) Brain cells of NOTCH3‐R170C and ‐WT mice (16 weeks of age) were subjected to single cell RNA sequencing (scRNAseq). (A) Left: Merged Uniform manifold approximation and projection (UMAP) plot representing 15 color‐coded cell clusters identified in the combined single‐cell transcriptomes. Cluster names were manually assigned. Right: Feature plots of Notch3 . (B) Expression of Notch3 among brain cells. (C) Violin plots of Notch3 and Aqp4 expression in astrocytes. (D) Brain protein of NOTCH3‐R170C or ‐WT mice (24 weeks of age) was subjected to western blot to evaluate AQP4 expression. N = 3 in each group. ** p < 0.01; by Student's t test (mean ± SEM). (E, F) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were prepared. (E) Aqp4 mRNA level was evaluated with RT‐PCR. (F) AQP4 protein expression in whole cell and plasma membrane of astrocytes was assessed with western blot. Experiments were repeated for three times. * p < 0.05; by Student's t test. Difference of means (± SEM) is displayed. (G) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were subjected to BN page and then 2D SDS page to evaluate the orthogonal arrays of particles (OAPs) of AQP4. (H) Coronal brain sections of NOTCH3‐R170C or ‐WT mice (24 weeks of age) were subjected to immunostaining of GFAP (green) and AQP4 (red). The AQP4 polarization was calculated by AQP4 perivascular endfeet area. White arrowheads indicated the depolarized AQP4 distribution. N = 6 in NOTCH3‐WT group and N = 5 in NOTCH3‐R170C group. * p < 0.05; by Student's t test (mean ± SEM).
    Figure Legend Snippet: Diminished AQP4 expression in NOTCH3‐R170C astrocyte. (A–C) Brain cells of NOTCH3‐R170C and ‐WT mice (16 weeks of age) were subjected to single cell RNA sequencing (scRNAseq). (A) Left: Merged Uniform manifold approximation and projection (UMAP) plot representing 15 color‐coded cell clusters identified in the combined single‐cell transcriptomes. Cluster names were manually assigned. Right: Feature plots of Notch3 . (B) Expression of Notch3 among brain cells. (C) Violin plots of Notch3 and Aqp4 expression in astrocytes. (D) Brain protein of NOTCH3‐R170C or ‐WT mice (24 weeks of age) was subjected to western blot to evaluate AQP4 expression. N = 3 in each group. ** p < 0.01; by Student's t test (mean ± SEM). (E, F) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were prepared. (E) Aqp4 mRNA level was evaluated with RT‐PCR. (F) AQP4 protein expression in whole cell and plasma membrane of astrocytes was assessed with western blot. Experiments were repeated for three times. * p < 0.05; by Student's t test. Difference of means (± SEM) is displayed. (G) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were subjected to BN page and then 2D SDS page to evaluate the orthogonal arrays of particles (OAPs) of AQP4. (H) Coronal brain sections of NOTCH3‐R170C or ‐WT mice (24 weeks of age) were subjected to immunostaining of GFAP (green) and AQP4 (red). The AQP4 polarization was calculated by AQP4 perivascular endfeet area. White arrowheads indicated the depolarized AQP4 distribution. N = 6 in NOTCH3‐WT group and N = 5 in NOTCH3‐R170C group. * p < 0.05; by Student's t test (mean ± SEM).

    Techniques Used: Expressing, RNA Sequencing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Clinical Proteomics, Membrane, SDS Page, Immunostaining

    Related Articles

    Blue Native PAGE:

    Article Title: NOTCH3 Mutation Causes Glymphatic Impairment and Promotes Brain Senescence in CADASIL
    Article Snippet: .. The protein content of the supernatant was diluted by 2X Blue Native PAGE Sample Buffer (Sangon Biotech, C506055‐0005). .. Eight percent polyacrylamide native gradient gels were prepared (Sangon Biotech, C631101‐0100).

    Article Title: NOTCH3 Mutation Causes Glymphatic Impairment and Promotes Brain Senescence in CADASIL.
    Article Snippet: .. The protein content of the supernatant was diluted by 2X Blue Native PAGE Sample Buffer (Sangon Biotech, C506055- 0005). .. Eight percent polyacrylamide native gradient gels were prepared (Sangon Biotech, C631101- 0100).



    Similar Products

    2x blue native page sample buffer  (Sangon Biotech)
    90
    Sangon Biotech 2x blue native page sample buffer
    Diminished AQP4 expression in NOTCH3‐R170C astrocyte. (A–C) Brain cells of NOTCH3‐R170C and ‐WT mice (16 weeks of age) were subjected to single cell RNA sequencing (scRNAseq). (A) Left: Merged Uniform manifold approximation and projection (UMAP) plot representing 15 color‐coded cell clusters identified in the combined single‐cell transcriptomes. Cluster names were manually assigned. Right: Feature plots of Notch3 . (B) Expression of Notch3 among brain cells. (C) Violin plots of Notch3 and Aqp4 expression in astrocytes. (D) Brain protein of NOTCH3‐R170C or ‐WT mice (24 weeks of age) was subjected to western blot to evaluate AQP4 expression. N = 3 in each group. ** p < 0.01; by Student's t test (mean ± SEM). (E, F) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were prepared. (E) Aqp4 mRNA level was evaluated with RT‐PCR. (F) AQP4 protein expression in whole cell and plasma membrane of astrocytes was assessed with western blot. Experiments were repeated for three times. * p < 0.05; by Student's t test. Difference of means (± SEM) is displayed. (G) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were subjected to <t>BN</t> <t>page</t> and then 2D SDS page to evaluate the orthogonal arrays of particles (OAPs) of AQP4. (H) Coronal brain sections of NOTCH3‐R170C or ‐WT mice (24 weeks of age) were subjected to immunostaining of GFAP (green) and AQP4 (red). The AQP4 polarization was calculated by AQP4 perivascular endfeet area. White arrowheads indicated the depolarized AQP4 distribution. N = 6 in NOTCH3‐WT group and N = 5 in NOTCH3‐R170C group. * p < 0.05; by Student's t test (mean ± SEM).
    2x Blue Native Page Sample Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2x+blue+native+page+sample+buffer/2x+blue+native+page+sample+buffer/pmc11760990-160-9-15
    Average 90 stars, based on 1 article reviews
    2x blue native page sample buffer - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Diminished AQP4 expression in NOTCH3‐R170C astrocyte. (A–C) Brain cells of NOTCH3‐R170C and ‐WT mice (16 weeks of age) were subjected to single cell RNA sequencing (scRNAseq). (A) Left: Merged Uniform manifold approximation and projection (UMAP) plot representing 15 color‐coded cell clusters identified in the combined single‐cell transcriptomes. Cluster names were manually assigned. Right: Feature plots of Notch3 . (B) Expression of Notch3 among brain cells. (C) Violin plots of Notch3 and Aqp4 expression in astrocytes. (D) Brain protein of NOTCH3‐R170C or ‐WT mice (24 weeks of age) was subjected to western blot to evaluate AQP4 expression. N = 3 in each group. ** p < 0.01; by Student's t test (mean ± SEM). (E, F) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were prepared. (E) Aqp4 mRNA level was evaluated with RT‐PCR. (F) AQP4 protein expression in whole cell and plasma membrane of astrocytes was assessed with western blot. Experiments were repeated for three times. * p < 0.05; by Student's t test. Difference of means (± SEM) is displayed. (G) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were subjected to BN page and then 2D SDS page to evaluate the orthogonal arrays of particles (OAPs) of AQP4. (H) Coronal brain sections of NOTCH3‐R170C or ‐WT mice (24 weeks of age) were subjected to immunostaining of GFAP (green) and AQP4 (red). The AQP4 polarization was calculated by AQP4 perivascular endfeet area. White arrowheads indicated the depolarized AQP4 distribution. N = 6 in NOTCH3‐WT group and N = 5 in NOTCH3‐R170C group. * p < 0.05; by Student's t test (mean ± SEM).

    Journal: CNS Neuroscience & Therapeutics

    Article Title: NOTCH3 Mutation Causes Glymphatic Impairment and Promotes Brain Senescence in CADASIL

    doi: 10.1111/cns.70140

    Figure Lengend Snippet: Diminished AQP4 expression in NOTCH3‐R170C astrocyte. (A–C) Brain cells of NOTCH3‐R170C and ‐WT mice (16 weeks of age) were subjected to single cell RNA sequencing (scRNAseq). (A) Left: Merged Uniform manifold approximation and projection (UMAP) plot representing 15 color‐coded cell clusters identified in the combined single‐cell transcriptomes. Cluster names were manually assigned. Right: Feature plots of Notch3 . (B) Expression of Notch3 among brain cells. (C) Violin plots of Notch3 and Aqp4 expression in astrocytes. (D) Brain protein of NOTCH3‐R170C or ‐WT mice (24 weeks of age) was subjected to western blot to evaluate AQP4 expression. N = 3 in each group. ** p < 0.01; by Student's t test (mean ± SEM). (E, F) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were prepared. (E) Aqp4 mRNA level was evaluated with RT‐PCR. (F) AQP4 protein expression in whole cell and plasma membrane of astrocytes was assessed with western blot. Experiments were repeated for three times. * p < 0.05; by Student's t test. Difference of means (± SEM) is displayed. (G) Primary NOTCH3‐R170C and ‐WT astrocyte cultures were subjected to BN page and then 2D SDS page to evaluate the orthogonal arrays of particles (OAPs) of AQP4. (H) Coronal brain sections of NOTCH3‐R170C or ‐WT mice (24 weeks of age) were subjected to immunostaining of GFAP (green) and AQP4 (red). The AQP4 polarization was calculated by AQP4 perivascular endfeet area. White arrowheads indicated the depolarized AQP4 distribution. N = 6 in NOTCH3‐WT group and N = 5 in NOTCH3‐R170C group. * p < 0.05; by Student's t test (mean ± SEM).

    Article Snippet: The protein content of the supernatant was diluted by 2X Blue Native PAGE Sample Buffer (Sangon Biotech, C506055‐0005).

    Techniques: Expressing, RNA Sequencing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Clinical Proteomics, Membrane, SDS Page, Immunostaining